This year “The Kentucky Academy of Science” event happened on 11th of November 2022. Several Berea student also presented their summer research. In the following we have the topic and the name of presenter from Berea college.
“Expression and Function of the Phagocytic Receptor, Jedi-I, in the Meninges” presented by Miss Tenzin Wangmo.
2. ” Confocal Microscopy reveals that SORLA Mediates Tau Endocytosis” presented by Miss Jenny Diaz.
3. “Constructing a Fluorescent Plasmid for Tagging Endogenous ITGa5 Locus in Skin Organoid Dermal Papilla Cells” presented by miss Jennan A. Lahamer
This summer Dr. Shrestha accompanied by several Berea college student, conducted research on exploring the key interactions of ATP synthase using site-directed mutagenesis, chemical modifications, and a battery of functional assays that may inform future antibacterial drug development.
According to the Centers for Disease Control and Prevention, there are at least 2.8 million infections and 35,000 deaths per year in the USA alone caused by antibiotic-resistant bacteria, and the WHO has estimated 10 million deaths worldwide by 2050. Over several years, bacteria responsible for common or severe infections have developed resistance to each new antibiotic coming to market. The success of the bedaquiline drug, which selectively inhibits the ATP synthase of Mycobacterium tuberculosis, has identified ATP synthase as an ideal target for the development of antibiotics. Additionally, the surge in drug-resistant bacterial infections necessitates the identification of novel drug target sites on ATP synthase. Therefore, I am interested in exploring the key interactions of ATP synthase using site-directed mutagenesis, chemical modifications, and a battery of functional assays that may inform future antibacterial drug development.
Dr. Mary Robert Garrett and her research team investigated the sustainable production of electron-deficient tetracenes(1), four-ringed, polyaromatic compounds, which have demonstrated promise as thermoelectric (TE) materials. This project examined an alternative approach to tetracenes (Scheme 1, below) that would lend itself to electron-deficient substrates in addition to electron-rich, and neutral substrates. Dr. Garret and her team successfully synthesized compound 5 from compound 2 via the three reported steps for an electron-rich substrate (R = CH3), electron-neutral substrate (R = H), and an electron-deficient substrate (R = F). The neutral product was synthesized in 20% yield, the electron-rich product in 49% yield and the electron-deficient product in 22% yield. As a result of this successful work, a new course has been developed for the Spring 2020 semester to continue this project in hopes of completing the fourth and final step realizing the only synthesis of electron-deficient substrates that also applies to electron-rich and neutral compounds. Upon that successful completion, the potential of these compounds to control electron flow in applications such as solar panels will be explored.
This summer, Berea College students conducted research at Berea College under the mentorship of Dr. Ron Rosen. The research that they performed was about factors that might lead to the observed lower intensities of infection of the Leuceruthrus micropteri, compared to Proterometra macrostoma, in their centrarchid fish definitive hosts. They collected samples at North Elkhorn creek, and then brought them back to the lab to experiment. When the cercaria freshly emerged, they put them in cold-blooded saline of different pH. The cercariae L. micropteri did not emerge from without pepsin in 2.0 and 2.5 pH. These results may partly explain the lower intensities of infection observed in centrarchid fish with L. micropteri when compared to P. macrostoma.